IL3-HE001 | 价格

IL3-HE001-100μg / 询价

IL3-HE001-500μg / 询价

IL3-HE001-500μgx2 / 询价

Human IL-33 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-33 Protein is expressed from E.coli without tag.
It contains Ser112-Thr270 [Accession | NP_254274.1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 18 kDa same as Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human IL-33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human IL-33 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human IL-33, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-1RL1, His Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human IL-33, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-33 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.

背景(Background)

Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.

分子别名(Synonyms)

C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2

文献(References)

(1)Liew FY, Girard JP, Turnquist HR. Interleukin-33 in health and disease. Nat Rev Immunol. 2016;16(11):676-689. doi:10.1038/nri.2016.95

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.